Core Facility Spotlight
Metabolite Profiling Facility
The Metabolite Profiling Facility located in Bindley Bioscience Center provides
state-of-the-art technologies that enable both qualitative and quantitative metabo-
lomics in complex biological systems.
An integral component of systems biology, along with genomics, transcriptomics
and proteomics, metabolite profiling helps define cellular functionality. Bindley’s
facility employs highly sensitive mass spectrometry coupled with liquid chromatog-
raphy and multi-dimensional gas chromatography for precise sample analysis.
» High throughput metabolite profiling with LC/MS, LC/MS-MS, GC/MS and
» Complete training in metabolite technologies
» Identification and characterization of small molecules
» Automated and manual sample preparation and Solid Phase Extraction (SPE)
» Metabolite methods development and consultation
» Bioinformatics consultation
» Various off-line HPLC separations and small molecule isolations
» Automated and manual computer search and analyses
» Waters Micromass LCT Premier (TOF), coupled to HPLC: the easy-to-use
benchtop mass spectrometer uses a high-resolution time-of-flight analyzer to
enable automated exact mass measurements.
» Agilent MSD/TOF, coupled to HPLC: the innovative analog-to-digital acqui-
sition system offers high resolution mass accuracy over a wide dynamic range.
» LECO Pegasus 4D GCxGC-TOF: the instrument can be operated as either a
GC/MS or a GCxGC/MS. When used in GCxGC mode, it is possible to sepa-
rate and detect thousands of compounds in a single sample.
» Agilent 6460 Triple Quadrupole: coupled with the Agilent 1200 Rapid Reso-
lution HPLC, the spectrometer provides highest sensitivity for small molecule
quantitation and MS/MS level analyses.
» Thermo-Finnigan Orbitrap: coupled with the Agilent 1100 HPLC system
with autosampler, the spectrometer provides a sub-dalton resolution trap
instrument for small molecule detection and quantification.
For more information on the Metabolite Profiling Facility, contact Bruce Cooper,
facility director, at 494-6282 or firstname.lastname@example.org. To reserve instrument time
or to schedule an appointment, contact Cooper or use the Resource Allocation Tool
Bruce Cooper, facility director of the Metabolite Profiling
Facility, discusses the laboratory’s expertise and instruments during Bindley Day in 2012.